Pearl Millets

Article Index

AICRP- Pear millet, JAU, CCS-HAU, SKNAU, MPKV, IARI (6)

Objectives:

  1. Development of pearl millet genotype for high Zinc and Iron through conventional breeding and identification of genomic regions associated with enhanced micronutrients through mapping and association mapping.
  2. Development of value added products for ready to cook and multi grain mixture suitable for mid-day meal scheme.
 

Target Traits

Iron > 42 ppm

Zinc > 32 ppm

                                                                                                                                                                                                              

Varieties released

 
  Hybrid/Variety Status CRP centers Iron (ppm) Zinc (ppm)
1 HHB 299 S. O 1379 (E)/ 27.03.2018 CCS HAU,Hisar 73 41
2 HHB 311 Identified CCS HAU, Hisar 83 39
3 RHB 233 Identified RARI, SKN AU 83 46
4 RHB 234 Identified RARI, SKN AU 84 41

 Common study across the partners (CRP Biofortification Parental Line Trial (CRPB PLT) Kharif 2017): The trial was successfully conducted across 6 locations viz., Mandor, Jamnagar, New Delhi, Dhule, Jaipur and Hisar in zone A and B during kharif 2017. The trial comprises 16 entries including two checks with high Fe and Zn contributed by different ICAR-AICRP on Pearl millet centers and ICRISAT. The performance of experimental entries along with two checks viz. ICMB 98222  and Dhanshakti for  days to flowering, plant height, panicle per plant, agronomic scores and Fe and Zn content (Table 1). High Fe content (84-118 ppm) were recorded in entry no. 3, 5, 4, 10, 8, 1 and high Zn content (57-84 ppm) were recorded in entry no. 3, 1, 5, 8, 1 and  4 in comparison to the best check Dhanshakti (Fe 83 and  Zn 55ppm).

Table 1: CRP Biofortification parental line trial (CRPB-PLT) kharif-17

 
 

Mandor

             Grand mean
Fe Zn Fe Zn Fe Zn Fe Zn Fe Zn Fe Zn Fe Zn
H 2302

47

 58 75 65 107 91 79 51 85 71 114 74 84 68
J-2571 40 38 78 46 105 75 88 41 68 30 107 59 81 48
10-SB-16 70 67 135 83 165 126 113 72 126 98 100 60 118 84
PPMI 952 60 45 114 58 135 76 105 51 96 61 96 54 101

57
PPMI 95865 65 49 115 56 161 108 104 44 100 69 120 71 111

66
PPMI 959 47 40 79 46 114 87 98 48 73 56 72 45 80

54
ICHFPT 16-4 46 43 86 58 101 97 102 46 38 8 86 54 77

51
ICHFPT 16-15 41 36 108 66 128 95 66 33 117 87 101 63 93

63
ICHFPT 16-17 25 22 44 28 47 49 49 26 37 29 74 54 46

35
ICHFPT 16-14 52 44 90 55 131 93 91 41 111 71 101 59 96

60
JMSB 20158 31 34 48 35 93 63 94 47 69 68 91 60 71

51
JMSB 20143 33 35 57 41 70 51 69 40 60 45 75 53 61

44
JMSB1 20159 29 30 72 42 83 62 93 40 70 55 79 50 71

46
JMSB1 20156 37 30 57 38 88 73 104 57 76 64 93 51 76

52
ICMB 98222 (C) 36 41 90 49 117 83 91 41 71 54 63 47 78

53
Dhanshakti (C) 43 37 83 50 123 97 87 40 80 49 84 55 83

55

 


 

ICAR-All India Coordinated Research Project (AICRP – PM) Pearl Millet, Jodhpur

PI: C Tara satyavathi

Composition and distribution of CRP Bio fortification Parental Line Trial (CRPB PLT) to associated centers was carried out. CRP Biofortification Parental Line Trial was done. Maintenance programme for 48 high Fe and Zn breeding lines including A, B and R line was carried out (Table 1). In R X R crossing programme, 67 crosses attempted with the use of 11 R lines having Fe content more than 100 ppm and 32 crosses were successful. Hybridization programme using 4 A lines and 10 R lines with high Iron and Zinc content was conducted. 5 A/B lines were maintained by paired crossing.

Table 1: Details of material used in maintenance programme of high Fe and Zn lines during Kharif 2017 at Mandor

 
S No. Name of Material Fe (ppm) Zn (ppm)
  R  Lines (38)    
1 DLBHI 1013-2    
2 ICJBT15-4    
3 ICJBT15-7    
4 ICMR 06555    
5 SGP 10-120-5    
6 HS 97/120-3    
7 PPMI 931    
8 PPMI 939    
9 PPMI 952* 111 67
10 PPMI 956 78 54
11 PPMI 957 82 54
12 PPMI 958* 106 66
13 PPMI 959* 91 64
14 H2302* 87 68
15 J-2571* 78 60
16 10-SB-16* 103 68
17 ICHFPT 16-4* 104 80
18 ICHFPT 16-14* 86 67
19 ICHFPT 16-15* 94 64
20 ICHFPT 16-17* 86 62
21 ICHFPT 16-1 91 56
22 ICHFPT 16-2 101 65
23 ICHFPT 16-2 100 69
24 ICHFPT 16-5 99 59
25 ICHFPT16-6 95 65
26 ICHFPT 16-7 98 65
27 ICHFPT16-8 106 69
28 ICHFPT 16-9 102 60
29 ICHFPT 16-10 95 64
30 ICHFPT 16-11 103 72
31 ICHFPT 16-12 103 64
32 ICHFPT 16-13 113 65
33 11-SB-16 84 60
34 26-SB-16 94 55
35 PPMI 951 83 59
36 PPMI 953 90 59
37 PPMI 954 88 55
38 PPMI 957 82 54
  Maintenance of A/B Pairs (5)    
1 JMSA 20143    
JMSB 20143* 65 46
2 JMSA 20156    
JMSB 20156* 69 51
3 JMSA 20158    
JMSB 20158* 76 56
4 JMSA 20159    
JMSB 20159* 86 60
5 ICMA 98222    
ICMB 98222*98 98

68

Note: * Being used in CRPBPLT 2017

Details of RXR crosses attempted during Kharif 2017 at Mandor

 
Female Male
PPMI 952  PPMI 958, 10-SB-16, ICHFPT 16-2, ICHFPT 16-3, ICHFPT 16-9, ICHFPT 16-11
PPMI 958  ICHFPT 16-2, ICHFPT 16-3, ICHFPT 16-11
10-SB-16  PPMI 958, ICHFPT 16-2, ICHFPT 16-3, ICHFPT 16-9, ICHFPT 16-11
ICHFPT 16-4  PPMI 952, PPMI 958, 10-SB-16, ICHFPT 16-2, ICHFPT 16-3, ICHFPT 16-9
ICHFPT 16-2  PPMI 958, ICHFPT 16-9, ICHFPT 16-11
ICHFPT 16-3  PPMI 958, ICHFPT 16-9
ICHFPT16-8  ICHFPT 16-9
ICHFPT 16-9  PPMI 958, ICHFPT 16-2, ICHFPT 16-3
ICHFPT 16-11  PPMI 958, ICHFPT 16-2
ICHFPT 16-13  PPMI 952

Details of AXR line crossing programme under CRP Biofortification project during Kharif 2017 at Mandor

 
A- Lines (4)   R-Lines (10)
JMSA 20143, JMSA 20156, JMSA 20158, JMSA 20159 X PPMI 952, PPMI 958, PPMI 959, 10-SB-16, J-2571, H2302, ICHFPT 16-4, ICHFPT 16-14, ICHFPT 16-17, ICHFPT 16-15

 


IARI, New Delhi

PI: Dr. S P Singh

Co PI: Dr S Mukesh Sankar

Thirty seven hybrids were developed by crossing Fe enriched parents and tested in Intermediate Station Trial. 12 hybrid combinations were found promising and selected for further evaluation. Fifty two hybrids were developed through hybridization of Iron and Zinc enriched parents.

Genetic linkage maps of seven Pearl millet linkage groups based on the F6:7 mapping   population derived from PPMI 683 x PPMI 627 for iron and zinc contents: Seven QTLs for iron and zinc contents for three consecutive years (2014, 2015 and 2016) at Delhi location detected by interval mapping on Pearl millet chromosome 3, chromosome 6 and chromosome 7 in the F6:7 population of the cross PPMI 683 x PPMI 627.

 

           

         

   Association mapping of 130 genotypes for grain iron and zinc content in Pearl millet     

 

AICRP-PM, CCS HAU, Hisar

PI: Dr. Devvrat Yadav

Forty two test crosses along with three checks were attempted and evaluated in CRP biofortification Station Trial during khaif 2017 were attempted and 14 hybrids selected for further evaluation in kharif 2018. Fifty two B X B crosses were evaluated for development of new seed parent and 11 crosses were selected for generating advanced F2 lines. One hundred three crosses (F1) were evaluated and 29 crosses were selected for further advancement to F2 generation. Parental screening for Polymorphism has been done and SSR marker analysis is in progress for one mapping population. In addition, protein content of dry stover was determined for 16 entries of CRP Biofortification Parental Line Trial.

 HIGH IRON CROSSES

 

 

Cross Combination

 

 

 

Fe (mg/kg)

 

XRF Value

AAS Value
HMS 16A
x
HB 15/085

84

79
HMS 16A
x
EBL12/237		 80 70
HMS 40A
x
HBL 1107		 82 81
ICMA 04999
x
HBL 1121		 88 76
SGP-10-120
x
HPT-2-12-15		 77 68
HFIT-3-11-125
x
HBL 1113		 78 65

 

HIGH ZINC CROSSES

 

 

 

Cross Combination

 

 

 

Fe (mg/kg)

 

XRF Value

AAS Value
HMS 16A     x    SGP-10-120

47

51
HMS 33A     x    LPBL-11-108 49 56

HMS 40A     x      HBL 1107

 50 48
HMS 41A    x    HFI-10-140 47 49
SGP-10-120     x    HPT-2-12-15 53 47
     

 

Under the project objective i.e. development of value added products, ready to cook and multigrain mixture suitable for Mid-day meal scheme, nutritional evaluation for crude protein, fat, ash, fibers and anti-nutrients (phytic acid and polyphenol) in ladoo, barfi and halwa, proximate composition of chapati, sev, dalia and khichri, protein iron zinc and calcium content of cake and biscuit, nutritional evaluation of processed and unprocessed pearl millet, organoleptic acceptability and self life study of pearl millet flour were carried out.

 In socio-impact study and popularization of biofortified products, various traditional products (ladoo, sev and dalia) and bakery products (cake and biscuits) were developed from biofortified variety of pearl millet (Dhanshakti) in combination with other food ingredients like semolina, bengal gram flour wheat grits and refined flour using standard recipes were studied for their organoleptic acceptability using nine point hedonic scale and it was in the range of ‘like moderately’.

AICRP-PM, RARI, JAIPUR, SKNAU


PI: Dr. L.D. Sharma


Based on high Fe and Zn content, sixteen hybrids were developed in summer 2017 and evaluated in kharif 2017. Ten hybrids having high Fe and Zn were evaluated in RBD design. Screening of 6 breeding lines (3 male sterile and 3 restorers) was carried out. Twenty lines were evaluated from material selected during Scientist Field Day kharif 2014 at ICRISAT. F2 of the 8 crosses were planted and 60 established restorer line have been sent to ICRISAT for quality parameter analysis including high Fe and Zn. A total of 9 entries contributed in coordinated trials and out of them 5 entries were in HT Fe & Zn trial.

 

 

 

 

 
SNo Hybrids identified
(under testing)		 Fe (ppm) Zn(ppm)
1 GHB 1157 78 35
2 GHB 1203 75 30
3 GHB 1151 71 31
4 GHB 1129 70 36
5 GHB 1038 70 28
6 GHB 1138 70 31
7 GHB 1225 76 50
8 GHB 1227 83 55

 

High Fe & Zn content CMS and B-lines identified at JAU

 

 
S.No. Name of Lines Fe (ppm) Zn (ppm)
1 258-KH-17 72 42
2 166-BC-17 96 60
3 182-BC-17 80 52
4 191-BC-17 107 72
5 242-BC-17 92 51
6 297-BC-17 97 50
7 JMSB 20101 89 57
8 JMSB 20102 72 62
9 JMSB 20142 86  61 
10 JMSB 20143  76 59 
11 JMSB 20151  114 66 
12 JMSB 20152  103 64 
13 JMSB 20154  102 66 
14 JMSB 20156  86  48
15 JMSB 20158  93  56
16 JMSB 20159  93  62
17 JMSB 20161 88 54

 

 High Fe & Zn content restorer lines at JAU 

 
S.No R lines Fe (ppm) Zn (ppm)
1 74-SB-17 78  43
 2  98-SB-17  83  53
 3  108-SB-17  74 46 
 4  39-SB-18  83 56 
5 41-SB-18 88 59
6 55-SB-18 115 71
7 57-SB-18 75 54
8 62-SB-18 78 66
9 64-SB-18   118 54 
10 65-SB-18 82 39
11 70-SB-18 109 60
12 75-SB-18 150 100
13 76-SB-18 123 71
14 77-SB-18 110 57
15 77-SB-18 70 63
 16  78-SB-18  120  55
17   79-SB-18  81  51
18   81-SB-18  95  61
19 J-2500 71 65
20 J-2526 69 60
21 J-2533 70 65
22 J-2552 85 57
23 J-2560 84 50
24 J-2565 70 55
25 J-2569 75 54
26 J-2571 94 53
27 J-2579 76 49
28 J-2587 77 64
29 J-2603 116 61
30 J-2605 83 48

 

 Publications:

  1. Anuradha N, Satyavathi CT, Bharadwaj C, Nepolean T, Sankar SM, Singh SP, Meena MC, Singhal T, Srivastava RK (2017) Deciphering genomic regions for high grain iron and zinc content using association mapping in pearl millet. Frontiers in Plant Science 8:412.doi: 10.3389/fpls.2017.00412 (NAAS Rating- 10.30)
  2. Anuradha N, C Tara Satyavathi, Meena MC, S MukeshSankar, Bharadwaj C, BhatJ, Singh O,Singh SP (2017) Evaluation of pearl millet [Pennisetumglaucum (L.) R. Br.] for grain iron and zinc content in different agro climatic zones of India. Indian Journal of Genetics and Plant Breeding77 (1)(NAAS Rating- 6.32)
  3. Berwal MK, Chugh LK, Goyal P, Kumar R,DevVart (2017) Protein, micronutrient,        antioxidant potential and phytate content of pearl millet hybrids and composites adopted for cultivation by farmers of Haryana, India. International Journal of Current Microbiology and Applied Sciences6(3): 376-386(NAAS Rating- 5.38)
  4. Anuradha N, Satyavathi CT, Bharadwaj C, MukeshSankar M, T Lakshmi Pathy TL (2018) Association of agronomic traits and micronutrients in pearl millet. International Journal of Chemical Studies 6(1): 181-184(NAAS Rating- 5.31)
  5. Anuradha N, Satyavathi CT, Bharadwaj C, Sankar M, Singh SP, Pathy TL (2018) Pearl millet genetic variability for grain yield and micronutrients in the arid zone of India. Journal ofPharmacognosy andPhytochemistry 7(1): 875-878(NAAS Rating- 5.21)
  6. DehinwalAK,YadavYP, Kumar A, Sivia SS (2017) Correlation and path coefficient analysis for different biometrical and harvest plus traits in pearl millet {Pennisetumglaucum(L.) R. Br.} Research in Environment and Life Sciences 10(5): 407-410(NAAS Rating- 3.74)
  7. Dube N, PC Mashurabad, F Hossain, R Pullakhandam, L Thingnganing, DK Bharatraj. 2018. β-Carotene bioaccessibility from biofortified maize (Zea mays) is related to its density and is negatively influenced by lutein and zeaxanthin. Food Funct. 2018 Jan 24;9(1):379-388. doi: 10.1039/c7fo01034f.

 

 

 

 

Copyrights reserve by @IIRR