Indira Gandhi Krishi Viswavidyalaya (IGKV), Raipur
PI: Dr Girish Chandel
Variety identified: R-RHZ-LI-23 (IET 25477) - Zinco Rice- MS developed by IGKV and identified by VIC 2018 for West Bengal, Chattisgarh and Odisha.
Evaluation of yield and grain protein content among rice genotype at IGKV (Chhattisgarh): A set of 15 rice genotypes containing 4 high yielding and gall midge resistant lines, two yield check, two high yielding germplasm of IGKV, six high grain protein content lines with one check for GPC were analyzed at four treatments of fertilizer dose (N:P:K) at the IGKV farm. Over all the higher grain yield was recorded in treatment number 3 i.e. @120kg N/ha across all rice genotypes. Out of 15 rice genotypes, CGR-77 showed highest GPC (11.32) in white/polished grain among all four treatments of NPK in comparison to high grain protein check IET -24780.
Screening of rice germplasm for identification of entries with high Zinc (≥ 26 µg/gm): A set of 192 rice germplasm were raised at farm of IGKV Raipur and screened for grain micronutrient level (Zn & Fe) in milled (polished) grains for two years i.e. 2016 – 2017. Among 192 germplasm lines analyzed, the variation observed for grain Zn content in the year 2017 ranged from 13.3 (GP145-45) to 39.7 (LALOO14) µg/gm whereas the variation observed for grain Fe content in the year 2017 ranged from 3.4 (VISHNU BHOG) to 22.0 {BANGLA GURMATIA (1756)} µg/gm. Based on the data obtained over the period of two years (2016 and 2017) total 9 rice landraces showing consistently higher grain zinc levels i.e. ≥ 26 µg/gm in polished grains were identified.
Molecular markers for zinc content: An indel marker targeting OsZIP10 gene was identified and was tested in a cross combination MTU1010/IR94033 and validated on a panel of rice germplasm.
Identification of markers associated with GPC trait: A panel of 192 rice germplasm was screened to determine the level of grain protein (GP) content in milled rice grains and to study the marker trait associations by using SSR markers. Protein content ranged from 4.82% to 11.11% for genotype GP145-45 and BAM766 (RCPL1-3C) respectively with an overall mean value of 7.04%. In total, 7 genotypes namely Kapoorsar - 9.48%, Lalmati - 10.15%, BAM698 (IC469345) - 9.56%, Nagina22 - 9.21%, BAM766 (RCPL1-3C) - 11.11%, Nipponbare - 9.17% and R 2270 - 9.47% were identified as high grain protein rice germplasm containing GP more than 9% among all germplasm lines analyzed. Association analysis was performed for the detection of relationship between the traits under study and the marker locus towards the identification of genetic counterparts of the traits. Total six markers were identified as highly significantly associated with the GP content of rice at p value< 0.01. Six significantly associated SSR markers with grain protein content were identified with P < 0.01viz. , RM204 (R2%: 20.02), RM118 (R2%: 5.22), RM125 (R2%: 18.43), RM230 (R2%: 8.47), RM105 (R2%: 8.76), and RM287 (R2%: 19.06).
Identification of transgressive variants for high zinc: From a cross between MTU1010 and IR94033 (F6), two lines viz MTIR-127 and MTIR-154 were identified for yield and high Zn concentration of 31.3 and 31.9 µg/gm respectively.
Tamil Nadu Agricultural University (TNAU), Coimbatore
PI: Dr P Jeya Prakash
Co PIs: Drs D Sudhakar, M Raveendran, S Rajeswari
Germplasm Screening: One hundred and fifty accessions consisting indigenous and exotic lines were analyzed for iron and zinc concentration. In brown rice, the iron concentration ranged from 7.7 µg/g (Varakkal) to 22.4 µg/g (IG 51) and zinc concentration ranged from 9.7 (IG 41) µg/g to 33.9 µg/g (Vadakathi samba). In polished rice, the iron concentration ranged from 3.6 µg/g (IR36) to 13.9 µg/g (Rama Kuruvaikar) and zinc concentration ranged from 8.1 µg/g (IR36) to 20.1µg/g (RPHP104).
Bi-parental Mapping population development: Based on the performance of genotypes in terms of zinc content, a set of genotypes were utilized in crossing programme to develop mapping population viz., Vadakathi samba, IG 39(EC 728779- 117750), IR 36 , CO(R)51 (High Yielding Variety), Kodai and RPHP 48.
Evaluation of protein and folate: Twelve high folate lines were identified. Folate content ranged from 25-30 micrograms/100g of whole grain in accessions namely CK275, Mappillai samba, Senkar, Murugankar and IR64.
Multiplication of high folate and protein genotypes: High folate and protein lines (Mapillai samba and CK 275) and low folate and protein lines (IG 29 and Katta samba) were raised in crossing block for further development of mapping population
Germplasm maintenance: A set of 150 genotypes used for association mapping panel has been raised for maintenance.
SNP discovery for iron, zinc, folate and protein in rice: Out of the available 150 germplasm including land races and improved varieties which have been phenotyped, 110 genotypes were employed for SNP discovery using Fluidgm SNP genotyping system. So far 96 SNP primers were used and results obtained. Further after running about 1000 SNP primers, analysis will be done for association mapping.