CCSHAU, Hisar Breeding for high Fe and Zn
PI: Dr S.K. Sethi
Co PI: Dr Vikram Singh
Development of breeding material:
- Biofortified Genotypes - WH 1080, WH 1063, WH 1127, WH 1136 (4)
- High Yielding Genotypes - WH 1142, HD 3086, DPW 621-50, DBW 88, WH 1105, WH 542 (6)
| WH 1127 | RL6043/4/NAC//PASTOR/3/BABAX |
| WH 1136 | NI 5663/RAJ 3765 |
| WH 1142 | CHEN AEGILOPS SQUARROSA(TAUS)//FCT/3/2*WEAVER |
| HD 3086 | DBW 14/HD 2733// HUW 468 |
| DPW 621-50 | KAUZ//ALTAR84/AOS/3/MILAN/KAUZ/4/HUITES |
| DBW 88 | KAUZ//ALTAR84/AOS/3/MILAN/KAUZ/4/HUITES |
| WH 1105 | MILAN/S87230//BABAX |
| WH 542 | JUP/BJY“S”//URES |
| WH 1063 | BARBET 1 Selection |
| WH 1080 | PRL/2*PASTOR |
Molecular characterization of parents: Forty-nine microsatellite markers linked with identified QTL for micronutrient were used for screening the parents. Out of 49, 30 SSRs were found polymorphic which amplified a total of 76 alleles with an average of 2.00 alleles per locus. The amplified PCR products ranged from 100-480bp.
Electrophoretic pattern of biofortified bread wheat parents and F1s in SSR analysis using primer xbarc 220, Lane 1- M-Ladder, G1-HD3086, G2-HD2967, G3-DBW88, G4-WH1080, G5-WH1063, G6-WH1142, G7-WH1127, G8-WH1136, G9-WH542, G10-DPW621-50, G11-WH1105
Using various combinations of crosses involving four biofortified genotypes and six high yielding genotypes, the following breeding material is being evaluated. Single ear selection in F4, back cross (BC1F3) and three way crosses (F3) generations will be done in 2018-19.
F4 generation: ~3700 (F3) agronomically superior plants with high Fe and Zn content
| WH1127 | WH1142, HD3086, DPW621-50, DBW88, WH1105, WH542 |
| WH1136 | WH1142, HD3086, DPW621-50, DBW88, WH1105, WH542 |
| WH712 | WH1142, HD3086, DPW621-50, DBW88, WH1105, WH542 |
| WH 1080 | WH1142, HD3086, DPW621-50, DBW88, WH1105, Wh542 |
| WH1063 | WH1142, HD3086, DPW621-50, DBW88, WH1105, WH542 |