ICAR-NRCPB, New Delhi
PI: Dr P K Mandal
Co PI: Dr S K Sinha
Amylase trypsin inhibitors in wheat:Grain development in wheat is very important and crucial process in crop life cycle and amylase-trypsin inhibitors (ATI) are a type of defence protein expressed only during grain development.
Expression analysis of ATI genes during grain filling: Previously, relative expression of CM3 ATI in 5 different genotypes was shown considering actin as an internal control in four different stages after anthesis i.e. 2, 3, 4 and 5 WAA (weeks after anthesis). During the reporting period the other different ATIs were also analyzed, but in 2 different varieties/accessions of each genome.
| Species | Genome | Variety/Accession No. |
| T. monococcum | AA | 104, 19 |
| Ae. speltoides | BB | P-84, P-83 |
| A. squarrosa | DD | P-95, P-94 |
| T. durum | AABB | PDW233, HI8428 |
| T. aestivum | AABBDD | PBW 343, HD3086 |
Genotypes including three diploid progenitors of wheat used for ATI expression studies: CM3 shows similar result for the other varieties/accessions of different genotypes (Fig.1). It shows higher expression in T. monococcum (AA), Ae. speltoides (BB), PDW 233 (AABB) at the end of the grain filling as the other genotypes i.e. Ae. squarossa (DD), PBW343 (AABBDD) shows less expression at the end of the grain filling. The analysis shows that hexaploid wheat shows higher expression of ATI in early stages during grain development. The tetraploid wheat shows higher expression in later stages of grain filling.
Relative expression of ATI CM3 among the different genotypes at different growth stages.
A: Comparative expression during development in each genotype separately;
B: Comparative expression among the genotypes in each growth stage separately.
Similarly the other important ATIs was also analyzed and showed different expression pattern in different genotypes at different growth stages.
Relative expression of ATI CM16 among the different genotypes at different growth stages.
A: Comparative expression during development in each genotype separately;
B: Comparative expression among the genotypes in each growth stage separately
Relative expression of ATI 0.19 among the different genotypes at different growth stages. A: Comparative expression during development in each genotype separately; B: Comparative expression among the genotypes in each growth stage separately
Relative expression of ATI CM2 among the different genotypes at different growth stages. A: Comparative expression during development in each genotype separately; B: Comparative expression among the genotypes in each growth stage separately
