IARI-Regional Station Indore
PI: Dr S Sai Prasad
Co PIs: Drs JB Singh and Rahul M Phuke
New Crosses Attempted: 55 crosses (30 durum and 25 bread wheat) were attempted for quality improvement among diverse parents.
| Durum Wheat | Donors: HI 8777, HI 8759, PDW 233, WH 896, UAS 462, HI 8737, HI 8713 |
| Recipients: HI 8663, HI 8591, HD 4672, | |
| Bread Wheat | Donors: WB02, HI 1605, 7th HPYT 403, 7th HPYT 422, 7th HPYT 425, 7th HPYT 426, 7th HPYT 447, 7th HPYT 443 |
Durum Quality Breeding Material: 466 lines for quality breeding material were maintained under high fertility and restricted irrigation conditions. Based on visual observations, 84 bulks and 896 single plants were selected and gain quality traits are under analyses.
CRP Multilocation Trial: A combined trial of advanced genotypes from IARI, RS Indore and UAS, Dharwad was conducted at IARI, RS Indore and UAS, Dharwad for 12 genotypes with 2 replications. Morphological, agronomic and grain yield characteristics were recorded, and data is in progress for post-harvest and quality traits viz., yellow pigment, protein content and grain Fe and Zn.
| Sr No | Genotype | Details of the genotypes |
| 1 | UASD-01 | DDK-50383 (Dicoccum) |
| 2 | UASD-02 | DDK-50404 (Dicoccum) |
| 3 | UASD-03 | UAS-428 (Durum check) |
| 4 | UASD-04 | DDK-50366 (Dicoccum) |
| 5 | UASD-05 | DDK-50391 (Dicoccum) |
| 6 | IND -01 | IND- 493 (Bread wheat) |
| 7 | IND-02 | IND- 496 (Bread wheat) |
| 8 | IND-03 | IND-505 (Bread wheat) |
| 9 | IND-04 | ID-1706 (Durum wheat) |
| 10 | IND-05 | 47th IDYN717 (Durum wheat) |
| 11 | IND-06 | MACS-2971 (Check variety) |
| 12 | IND-07 | HI- 8777 (Check variety) |
Quality Trial for multiple sowing conditions: 20 genotypes (10 durum and 10 bread wheat) were sown for quality analysis in three different growing conditions viz., early sown, timely sown and late sown. Morphological, agronomic and grain yield characteristics was recorded, and data is in progress for post-harvest and quality traits viz., yellow pigment, protein content and grain Fe and Zn.
| Sr No | Genotype | Sr No | Genotype |
| 1 | HI 8759 | 11 | HI 8777 |
| 2 | HI4728 | 12 | HI 8713 |
| 3 | HI 8737 | 13 | HI 8627 |
| 4 | HI 8663 | 14 | PDW 233 |
| 5 | HI 8498 | 15 | WH 896 |
| 6 | HI 1544 | 16 | HI 1605 |
| 7 | GW 322 | 17 | HI 1612 |
| 8 | GW 366 | 18 | DBW 110 |
| 9 | WB-02 | 19 | MP 3288 |
| 10 | HD 2967 | 20 | HI 1531 |
Soil Sample Analysis
The soil samples from the fields of CRP-biofortification and multiple sowing conditions were collected from their respective IARI-Indore fields. They were analyzed for available iron and zinc content; and found that Fe and Zn contents in soil were in sufficient range required for normal plant growth requirements.
| Sr No | Sample ID | Fe (g/kg) | Zn (g/kg) |
| 1 | Indore I | 39.47 | 47.52 |
| 2 | Indore II | 46.94 | 48.70 |
| 3 | Indore III | 41.88 | 48.56 |
Generation Mean analysis Trial: Six generations i.e., P1, P2, F1, F2, BC1 and BC2 for three cross combinations were conducted to study the gene action for grain yield as well as quality traits like grain Fe, Zn and other quality traits. All the above three trials will be continued for one more year for evaluation of G ×E interactions for their performance.
Molecular Characterization of Quality Breeding material: Attempts were made to validate SSR markers linked to high yellow pigments viz., SCAR 807, YP7A Xgpw4050, Xbarc84, Xcfa2240, Xgwm129 and Xgwm150 in high and low yellow pigment durum genotypes. Eight genotypes were amplified using SCAR 807 marker among which five genotypes viz., NIDW 73, Jay, Meghdoot, B-206 and PDW 233 have shown amplification of single band at 465 bp, and two genotypes viz., HI 8713 and WH 896 amplified both the phytoene synthase bands (465 bp and 967 bp). The two genotypes HI 8713 and WH 896 with high yellow pigment content showed positive amplicon for phytoene synthase. Another SSR marker YP7A linked to phytoene synthase on chromosome 7A was used to amplify four durum wheat genotypes viz., Meghdoot, HI 8498, HI 8713 and WH 896. The PCR amplification showed single amplicon for Meghdoot at ~ 200bp and two amplicons for HI 8713 and WH 896 (~ 200bp and between 300-400bp). The two durum wheat genotypes HI 8713 and WH 896 showed positive alleles for both the yellow pigment linked molecular markers i.e. SCAR 807 and YP7A; and also high yellow pigment content and hence these genotypes will be used as donors for improving yellow pigment in durum wheat.
Fifty three stable durum lines from high fertility and restricted irrigation conditions quality breeding material were screened with SCAR 807 (Dominant SSR marker) linked to high yellow pigment and among 53 lines, 39 lines showed amplicon for high yellow pigment, hence SCAR 807 marker can be used for early generation quality breeding material screening.